A sensitive HPLC-DMS/MS/MS method for multiplex analysis of androgens in human serum without derivatization and its application to PCOS patients

•Development of a new HPLC-DMS/MS/MS method to determine androgens in human serum.•A sensitive and specific quantification method for multiplexing androgens.•Resolution increase of isobaric compounds and reduction of baseline noise.•Successful validation of the method and its application to clinical sample assay.