High-throughput genotyping of high-homology mutant mouse strains by next-generation sequencing
Methods(2021)
摘要
•Next generation sequencing is a scalable solution to genotyping mutant mice.•Ratios of wild type and mutant sequence counts are used to call the genotype.•Hundreds of samples can be multiplexed into one sequencing experiment.•Amplification of high-homology genes can be easily filtered out during analysis.
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关键词
CRISPR,het,hom,NGS,NHEJ,PCR,QC,sgRNA,WT
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