Systematic monitoring of glanders-infected horses by complement fixation test, bacterial isolation, and PCR.

Diego Candido Abreu, Aline Silva Gomes, Danielle Klein Tessler,Daniela Pontes Chiebao,Cláudia Del Fava,Adriana Hellmeister de Campos Nogueira Romaldini, Mateus Carvalho Araujo,Júlio Pompei, Guilherme Figueiredo Marques,Ricardo Harakava,Edviges Maristela Pituco,Alessandra Figueiredo de Castro Nassar

Veterinary and Animal Science（2020）

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摘要

•A modified PCR protocol improved the diagnostic sensibility for glanders.•Horses with chronic glanders disease may show false negative serology results.•The cold CFT is recommended in Brazilian equine health program.•Burkholderia mallei isolation and detection were performed in São Paulo state, Brazil.

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antigen,Burkholderia mallei,incubation temperature,sero-diagnosis

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