Preparation of Mammalian Nascent RNA for Long Read Sequencing.
Current protocols in molecular biology(2020)
摘要
Long read sequencing technologies now allow high-quality sequencing of RNAs (or their cDNAs) that are hundreds to thousands of nucleotides long. Long read sequences of nascent RNA provide single-nucleotide-resolution information about co-transcriptional RNA processing events-e.g., splicing, folding, and base modifications. Here, we describe how to isolate nascent RNA from mammalian cells through subcellular fractionation of chromatin-associated RNA, as well as how to deplete poly(A)
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关键词
chromatin,fractionation,long read sequencing,nascent RNA
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