Development of 49 novel microsatellite markers from Next-generation sequencing data and a robust method for parentage tests in the emu (Dromaius novaehollandiae)

Yuichi Koshiishi,Hironobu Uchiyama,Michiko Murata-Okubo,Keisuke Tanaka,Yuichi Kameyama,Hiroki Hirayama,Kenta Wada

Gene（2021）

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摘要

•Using NGS data, 49 novel SSR markers for the emu were developed.•Dinucleotide motif (AC)n was the most frequently detected in the emu genome.•A robust parentage test using a 12 SSR marker set was established for Japanese emu.

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关键词

SSR,NGS,PIC,BLAST,NA,HE,HO,GPR1,ST8SIA4,COPS7B

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