Topology engineering via protein catenane construction to strengthen an industrial biocatalyst

Qiang Zheng,Mengjiao Wang,Lin Zhang,Waqas Ahmad,Hongxia Li,Yigang Tong,Guojun Zheng,Shaozhou Zhu

Journal of Biotechnology（2021）

引用 8|浏览11

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摘要

•Two different protein catenanes were constructed for an industrial biocatalyst.•Protein catenation enhanced both the thermo- and proteolytic stabilities of gamma lactamase.•Catenated enzymes displayed higher enzyme efficiencies (Kcat/Km).

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关键词

Topology engineering,Protein catenanes,Vince lactam,gamma lactamase,SpyTag/SpyCatcher

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