Pedobacter puniceum sp. nov. Isolated from Sludge

Strain HX-22-1 T was isolated from the sludge collected from the outlet of the biochemical treatment facility of an agricultural chemical factory in Nanjing city, Jiangsu province, PR China. Strain HX-22-1 T is Gram-staining-negative, rod-shaped, non-spore-forming and non-flagellated. The 16S rRNA gene-based phylogenetic analysis indicates that the strain HX-22-1 T belongs to the genus Pedobacter , closely related to Pedobacter glucosidilyticus KCTC 22438 T (98.63% similarity). The genomic DNA G+C content of the strain was 34.4 mol%. Strain HX-22-1 T was able to grow at 16–37 °C (optimum at 30 °C), at pH 6.0–8.0 (optimum at pH 7.0), and with 0–1% (w/v) NaCl (optimum at 0). Predominant fatty acid constituents were iso-C 15:0 and summed feature 3 (iso-C 16:1 ω7c and/or C 16:1 ω6c ). The predominant respiratory ubiquinone was MK-7. The polar lipid profile is composed of phosphatidylethanolamine (PE), aminophospholipid (APL), aminolipid (AL), and phospholipids (PL). The ANI and dDDH values obtained between the genomes of HX-22-1 T and P . glucosidilyticus KCTC 22438 T were 89.6 and 38.8%, respectively. On the basis of data from phenotypic, chemotaxonomic, and genotypic analysis, strain HX-22-1 T represents a novel species of the genus Pedobacter , for which the name Pedobacter puniceum sp. nov. is proposed. The type strain is HX-22-1 T (= KCTC 72655 T = CCTCC AB 2019348 T ).