MICROBIOTA OF PURULENT NECROTIC LESIONS IN PATIENTS WITH DIABETIC FOOT SYNDROME

The Diabetic Foot Syndrome (DFS) is one of the most serious complications of the diabetes mellitus (DM). Infections play a minor role in the genesis of a diabetic foot lesion but assume considerable importance in existing injuries as a risk factor for progression to amputation. Optimal treatment of these diabetic foot infections requires recognizing which foot ulcers are infected and prescribing pathogen-appropriate antibiotic therapy. The main goal of this study was the identification of microbiota's structure of purulent-necrotic lesions in 34 patients with a severe form of DFS. The certain amount of affected area was taken as material for research. The microbial composition was identified by sequencing of DNA fragments of 16S rRNA gene including V3 and V4 variable sequences. As a result, the microbiota of purulentnecrotic lesions was characterized by poly-microbial association. The high degree of the anaerobe bacteria which are generally responsible for the development of intrahospital infectious diseases was observed.