The Impact Of Bioactive Surfaces In The Early Stages Of Osseointegration: An In Vitro Comparative Study Evaluating The Hanano (R) And Slactive (R) Super Hydrophilic Surfaces

There is an increased effort on developing novel and active surfaces in order to accelerate their osteointegration, such as nanosized crystalline hydroxyapatite coating (HAnano (R)). To better understand the biological behavior of osteoblasts grown on HAnano (R) surface, the set of data was compared with SLActive (R), a hydrophilic sandblasted titanium surface. Methodologically, osteoblasts were seeded on both surfaces up to 72 hours, to allow evaluating cell adhesion, viability, and set of genes encoding proteins related with adhesion, proliferation, and differentiation. Our data shows HAnano (R) displays an interesting substrate to support cell adhesion with typical spread morphologic cells, while SLActive (R)-adhering cells presented fusiform morphology. Our data shows that the cellular adhesion mechanism was accompanied with upexpression ofintegrin beta 1,Fak, andSrc, favoring the assembling of focal adhesion platforms and coupling cell cycle progression (upmodulating ofCdk2,Cdk4, andCdk6genes) in response to HAnano (R). Additionally, both bioactive surfaces promoted osteoblast differentiation stimulus, by activatingRunx2,Osterix, andAlpgenes. Although both surfaces promotedRanklgene expression, Opg gene expression was higher in SLActive (R) and this difference reflected on theRankl/Opgratio. Finally, Caspase1 gene was significantly upmodulated in response to HAnano (R) and it suggests an involvement of the inflammasome complex. Collectively, this study provides enough evidences to support that the nanohydroxyapatite-coated surface provides the necessary microenvironment to drive osteoblast performance on dental implants and these stages of osteogenesis are expected during the early stages of osseointegration.