Organoid Easytag: an efficient workflow for gene targeting in human organoids

Human organoid systems recapitulate key features of organs offering platforms for modelling human developmental biology and disease. However, tissue-derived organoids suffer from low efficiency of genetic manipulations, especially CRISPR/Cas9-mediated knock-ins. We have systematically optimised and developed an “Organoid Easytag” pipeline for efficient (40-65%) and accurate gene targeting to facilitate generation of reporter lines and gene knock-outs in organoid based research.