Bridging scales in scattering tissues via multifocal two-photon microscopy

Imaging biological systems at subcellular resolution and across scales is essential to understanding how cells form tissues, organs, and organisms. However, existing large-scale optical techniques often require harsh tissue-clearing methods that compromise the integrity of the cell membrane, cause significant morphological changes, and reduce the signal of fluorescent proteins. Here, we demonstrate multifocal two-photon microscopy that allows imaging mesoscopic scattering tissues at high resolution and high speed.