Single cell visualisation of mir-9a and Senseless co-expression during Drosophila melanogaster embryonic and larval peripheral nervous system development
biorxiv(2020)
摘要
The peripheral nervous system (PNS) comprises the sensory organs that allow the fly to detect environmental factors such as temperature and pressure. PNS development is a highly specified process where each sensilla originates from a single sensory organ precursor (SOP) cell. One of the major genetic orchestrators of PNS development is , which encodes a zinc finger transcription factor (Sens). Sens is both necessary and sufficient for SOP differentiation. expression and SOP number are regulated by the microRNA . However, the reciprocal dynamics of and are still obscure. By coupling smFISH with immunofluorescence, we are able to visualize transcription of the locus and expression of Sens simultaneously. During embryogenesis, we show that the expression of in SOP cells is rapidly lost as expression increases. However, this mutually exclusive expression pattern is not observed in the third instar imaginal wing disc, where some -expressing cells show active sites of transcription. These data challenge and extend previous models of regulation, and show complex co-expression dynamics between and . The differences in this dynamic relationship between embryonic and larval PNS development suggest a possible switch in function. Our work brings single-cell resolution to the understanding of dynamic regulation of PNS development by and .
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关键词
MicroRNA,<italic>mir-9a</italic>,<italic>Senseless</italic>,Peripheral Nervous System,embryogenesis,Wing Disc
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