Production method of human multipotent stem cells for simultaneously preventing cell ageing and malignant transformation

The invention discloses a production method of NRF2 gene-enhanced mesenchymal stem cells for simultaneously preventing cell ageing and malignant transformation The method comprises the following steps: successfully reconstructing an anti-adversity related gene by using a gene editing technology for the first time, and modifying only one coding site to realize controllable active activation of an endogenous anti-adversity gene to obtain gene-enhanced mesenchymal stem cells with improved anti-adversity. In vivo and in vitro experiments verify that the gene-enhanced stem cells can prevent adversity and cell ageing, and have good tissue restoration function and malignant tumor transformation resisting ability. The method also solves two key and bottleneck problems of the effectiveness and the safety in cell transplantation therapy.