STUDY ON THE MECHANISM OF MIR-1254 PROMOTING PROLIFERATION AND MIGRATION OF GASTRIC CANCER BY TARGETING NKD1

Objective: To investigate the effect of miR-1254 on proliferation and migration of gastric cancer cells. Methods: The expression of miR-1254 in gastric cancer tissues and cells was investigated by TCGA database analysis and real-time quantitative PCR. After overexpression or knockout of miR-1254 in gastric cancer cells, the effect of miR-1254 on the proliferation of gastric cancer cells was detected by CCK-8 assay, and the effect of miR-1254 on the migration of gastric cancer cells was measured by scratch method. The binding sequence of miR-1254 and NKD1 3'UTR was predicted by TargetScan software, and the regulatory effect of miR-1254 on NKD1 was further verified by luciferase reporter gene detection. The effect of miR-1254 on the expression of NKD1 in gastric cancer cells was determined by western blot assay. The expression of NKD1 in tumor tissues and normal tissues was analyzed by immunohistochemistry method, and the effect of over-expression of NKD1 on the proliferation and migration of gastric cancer cells was investigated. Finally, rescue experiments were used to further verify whether miR-1254 affects the proliferation and migration of gastric cancer cells by regulating NKD1. Results: Compared with normal tissues and normal gastric epithelial cells, the expression of miR-1254 in human gastric cancer tissues and gastric cancer cells was increased (P < 0.01). Over-expression of miR-1254 in SGC7901 cells can enhance the proliferation and migration of gastric cancer cells (P < 0.01). Moreover, the inhibition of miR-1254 expression in AGS cells can decrease the proliferation and migration ability of gastric cancer cells (P < 0.01). MiR-1254 can combine with NKD1 3'UTR region, thus reducing the expression of NKD1. Over-expression of NKD1 could inhibit the proliferation and migration of gastric cancer cells (P < 0.01), and at the same time, it could weaken the enhancement of proliferation and migration of gastric cancer cells induced by over-expression of miR-1254 (P<0.01). Conclusion: MiR-1254 is capable of promoting proliferation and migration of gastric cancer cells by inhibiting the expression of NKD1.