INHIBITION OF RESVERATROL ON BLADDER CANCER AND ITS MOLECULAR MECHANISM

Objective: It is to investigate the clinical effect of serum CEA, CA153, CYFRA21-1, CA125, NSE, and CA199 in the application of Myrian imaging rTo investigate the inhibition of resveratrol on the in vitro proliferation activity of human bladder cancer 5637 cells and its possible molecular mechanism. Methods: Human bladder cancer 5637 cells were cultured in vitro and divided into a blank control group and resveratrol groups (25 mu mol/l, 50 mu mol/l, 100 mu mol/l and 200 mu mol/l); then, the effect of resveratrol on the proliferation of 5637 cells was detected by conducting MTT tests. The effect of resveratrol (200 mu mol/l) on the apoptosis of 5637 cells was detected by flow cytometry. In the blank control group and resveratrol (25 mu mol/l, 50 mu mol/l, 100 mu mol/l and 200 mu mol/l) groups, total protein and mRNA were extracted. The effect of resveratrol on the expression of p-Akt and PTEN protein and mRNA were detected by Western blot and PCR. Results: Resveratrol significantly inhibited the in vitro proliferation activity of 5637 cells in a dose-dependent and time-depend-ent manner. The flow cytometry results showed that resveratrol induced apoptosis and arrested the cell cycle in G1 phase in 5637 cells. Resveratrol significantly inhibited the expression of p-Akt mRNA and protein and upregulated the expression level of PTEN mRNA and protein compared with the control (p < 0.05); these differences were statistically significant. Conclusion: Resveratrol can inhibit the proliferation of bladder cancer cells and induce apoptosis by the upregulation of PTEN expression.