Rapid separation of lipoproteins based on microfluidic chip electrophoresis

A rapid assay of separating lipoproteins with microfluidic chip is described. The pre-stained lipoproteins with nitrobenzoxadiazole-6-ceramide were separated and analysed in quartz chip with laser induced fluorescence (LIF) detector. The electrophoresis buffer was accomplished with 40 mmol/L tricine buffer by addition of 40 mmol/L methylglucamine. After 40 s sample injection by applying 500 V voltage, the 2000 V separation voltage was switched on, and the peaks of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were detected within 2 min. After calculation coefficient of variation (CV) of sample migrating time was not more than 2.6% through 5 times detection. The method was sensitive, rapid, simple and reproducible for diagnosis of hyperlipoidemia.