Correction to: Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L.

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY(2021)

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摘要
A novel Gram-positive and endospore-forming bacterium assigned as strain SPB7 T which is also a new source of a cyclic diketopiperazine (3S,6S)-3,6-diisobutylpiperazine-2,5-dione is described. A polyphasic (biochemical, phenotypic and genotypic) approach was used to clarify the taxonomic affiliation of this strain. The partial and complete 16S rRNA gene sequences revealed that strain SPB7 T is a member of the Bacillus genus [showing high similarity (> 98.70%) with Bacillus s pizizenii NRRL B-23049 T , Bacillus tequilensis KCTC 13622 T , Bacillus inaquosorum KCTC 13429 T and Bacillus cabrialesii TE3 T ]. The maximum values for average nucleotide identity (ANI) and in silico DNA–DNA hybridization (GGDC, Formula 2) of strain SPB7 T was obtained for twenty-five strains of Bacillus s pizizenii (ANI 95.01–95.48% and GGDC 62.70–60.00%). The whole-genome phylogenetic relationship showed that SPB7 T formed an individual and separated clade with the Bacillus s pizizenii group. Principal cellular fatty acids identified in strain SPB7 T were anteiso C 15:0 , anteiso C 17:0 , iso C 15:0 , iso C 17:0 , C 16:0 , C 10:0 3OH and iso C 17:1 ϖ10c . Polar lipid profile showed presence of diphosphotidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and five unknown lipids. Cells were rod shaped, catalase, oxidase-positive and motile. Growth occurred at 20–45 °C (optimal 35 °C), at pH 6.0–10.0 (optimal pH 8) and 0–10% (w/v) NaCl (optimal 2%). The phenotypic, biochemical, and genotypic traits of strain SPB7 T strongly supported its taxonomic affiliation as a novel species of the Bacillus genus, for which the name Bacillus rugosus sp. nov. is proposed. The type strain is SPB7 T (= NRRL B-65559 T , = CICC 24827 T , = MCC 4185 T ).
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关键词
Average nucleotide identity (ANI), Bacillus rugosus, DNA-DNA hybridization, Genome-to-genome distance calculator (GGDC), Marine sponge, Whole-genome phylogenetic identification
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