Moxifloxacin efficacy against Mycobacterium abscessus in vivo using zebrafish model

Moxifloxacin (MFX) showed good activity against () and was suggested as one of the antibiotic regimens for adults with disease. However, some other studies showed that MFX showed less or none activity against . In our study we aim to evaluate MFX activity against using zebrafish (ZF) model . MIC of each drugs were determined by broth microdilution method. labeled by CM-DiI, were micro-injected into ZF. Survival curves were determined by recording dead ZF every day. After 4 days of incubation ZF were lysed. Colony-forming unit (CFU) were enumerated and results are expressed as mean log10 CFU per ZF. Bacteria dissemination and fluorescence intensity in ZF were observed and analyzed. Inhibition rate was also calculated. In our study MFX showed good activity . But MFX showed limited restriction to . The association between increased survival and high dose of MFX is not significant. Same results were observed in bacterial fluorescence intensity and inhibition rates, with no significant difference when compared with no drug group (P > 0.05). However, significant difference was observed in azithromycin (AZM) group. MFX showed limited efficacy on using ZF model. MFX’s activity need to be confirmed.