EFFECT OF ALCOHOLIC EXTRACT OF ADHATODA VASICA L. LEAVES AND ALLIUM CEPA L. BULB IN DIABETIC WOUND HEALING IN WISTAR ALBINO RATS

Objective of the study was to evaluate the effect of alcoholic extract of Adhatoda vasica L. leaves (AV) and Allium cepa L. bulb (AC) in diabetic wound healing in Wistar albino rats. Forty eight Wistar albino rats (weight 250 +/- 50 g) of either sex were randomly divided into 8 groups. Groups 1-4, excision wound model (received drug orally till complete healing); Groups 5-8. incision and dead space model (received drug orally for 11 days). Group 1: Distilled water. Group 2: Glibenclamide 0.5 mg/kg. Group 3: AV 400 mg/kg. Group 4: AC 300 mg/kg. Group 5: Distilled water. Group 6: Glibenclamide 0.5 mg/kg. Group 7: AV 400 mg/kg. Group 8: AC 300 mg/kg. Percentage wound closure and re-epithelization measured in excision wound model. Tissue breaking strength measured in incision wound model. Dry weight granuloma (weight of dried granulation tissue), hydroxyproline estimation and histopathology examination was done in dead space wound model. Significant improvement in wound closure on day 3, 7 and 11. re-epithelization on day 11, tissue breaking strength, dry weight granuloma and hydroxyproline content was seen in AV group. Significant improvement in wound closure on day 11, re-epithelization on day 11, tissue breaking strength, dry weight granuloma and hydroxyproline content was seen in AC group. In histopathology, fibroblast formation higher in Adhatoda vasica group and fibroblast formation, neoangiogenesis increased in Allium cepa group but not achieved statistically significant level. Alcoholic extract of Adhatoda vasica L. and Allium cepa L. improves wound contraction, re-epithelization, granulation tissue and collagen formation.