Supplement of nitric oxide through calcium carbonate-based nanoparticles contributes osteogenic differentiation of mouse embryonic stem cells.

•The effective delivery of S-nitrosothiol as a NO donor using calcium carbonate-mineralized nanoparticles into ESCs.•The osteoinductive effect of NO signaling molecule on ESCs.•Identifying intracellular NO-related signaling pathways through global gene expression analysis.•The management of intracellular NO levels using nanomaterials can improve the efficacy of stem cell differentiation.