Identification and characterization of a polyurethanase with lipase activity from Serratia liquefaciens isolated from cold raw cow's milk

Cleonice Aparecida Salgado,Felipe Alves de Almeida,Edvaldo Barros,Maria Cristina Baracat-Pereira,François Baglinière,Maria Cristina Dantas Vanetti

Food Chemistry（2021）

引用 17|浏览6

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摘要

•Serratia liquefaciens produces a lipase that has been identified as polyurethanase.•65 kDa alkaline polyurethanase shows the conserved domain of family I.3 lipase.•Assays with BME, DTT, PMSF, and DEPC confirm that it is a family I.3 lipase.•In silico, polyurethanase uses catalytic serine (GXSXG) to bind to substrates.

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关键词

p-nitrophenyl palmitate (p-NPP) – PubChem CID: 73891,p-nitrophenol (p-NP) – PubChem CID: 980,Methylumbelliferyl butyrate (MUB) – PubChem CID: 87247,Tosyl-l-phenylalanine chloromethyl ketone (TPCK) – PubChem CID: 4339647,β-mercaptoethanol (BME) – PubChem CID: 1567,Dithiothreitol (DTT) – PubChem CID: 446094,Ethylenediaminetetraacetic acid (EDTA) – PubChem CID: 6049,Phenylmethylsulfonyl fluoride (PMSF) – PubChem CID: 4784,Diethyl pyrocarbonate (DEPC) – PubChem CID: 3051

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