An LC-MS/MS analytical method for the determination of uremic toxins in patients with end-stage renal disease

•Development of LC-MS/MS method for determination of uremic toxins.•Quantification of 26 uremic toxins in serum samples of healthy subjects and patients with ESRD before and after hemodialysis.•Investigation of protein binding ratio of 26 uremic toxins.•Identification different class of markers for evaluating the effectiveness of hemodialysis.