A Tlr7 Agonist Activates Bovine Th1 Response And Exerts Antiviral Activity Against Bovine Leukemia Virus

Bovine leukemia virus (BLV) infection is a bovine chronic infection caused by BLV, a member of the genus Deltaretrovirus. In this study, we examined the immunomodulatory effects of GS-9620, a toll-like receptor (TLR) 7 agonist, in cattle (Bos taurus) and its therapeutic potential for treating BLV infection. GS-9620 induced cytokine production in peripheral blood mononuclear cells (PBMCs) as well as CD80 expression in CD11c(+) cells and increased CD69 and interferon (IFN)-gamma expressions in T cells. Removing CD11c(+ )cells from PBMCs decreased CD69 expression in T cells in the presence of GS-9620. These results suggest that TLR7 agonism promotes T-cell activation via CD11c(+ )cells. Analyses using PBMCs from BLV-infected cattle revealed that TLR7 expression in CD11c(+ )cells was upregulated during late-stage BLV infection. Furthermore, GS-9620 increased IFN-gamma and TNF-alpha production and inhibited syncytium formation in vitro, suggesting that GS-9620 may be used to treat BLV infection.