Serological Survey And Dna Screening Ofleptospiraspp. In Free-Living Adult Tufted Capuchin Monkeys (Cebus Apella Nigritus) In A Forest Reserve Southeast Sao Paulo State, Brazil

JOURNAL OF MEDICAL PRIMATOLOGY(2021)

引用 4|浏览6
暂无评分
摘要
Background Leptospirosis is an important anthropozoonosis. The study investigated the presence of anti-Leptospiraantibodies and detection ofLeptospiraspp DNA in the urine as well as the biochemical profile in Neotropical wild primates living in a forest reserve from Southeast Sao Paulo State, Brazil. Methods Blood samples were obtained from 50 adult tufted capuchin monkeys (Cebus apella nigritus). Urine samples were obtained only from male primates. The screening for antibodies againstLeptospiraspp was evaluated by microscopic agglutination test (MAT).LeptospiraDNA in the urine was evaluated by polymerase chain reaction (PCR) considering the target gene LipL32. Biochemical profile was evaluated by using a spectrophotometer. Results The MAT results included 39 (78%) serum reactive animals with the proportions of 28/39 males and 11/39 females. The most frequent reactive serogroups were Icterohemorrhagiae, Canicola, and Autumnalis. All urine samples were negative for leptospiral DNA. There were no significant differences between sexes for aspartate aminotransferase (AST) and alkaline phosphatase values, but alanine aminotransferase (ALT), creatinine, glucose, and urea were significantly higher in males. Conclusions Tufted capuchin monkeys were sera reactive against leptospirosis. Prevalence was similar for the 2 sexes. Leptospiral DNA was not detected in the urine of sera reactive primates tested by the MAT method. ALT, creatinine, glucose, and urea values were higher in male animals.
更多
查看译文
关键词
biochemical profile, Cebus apella nigritusmonkey, leptospirosis, microscopic agglutination test, polymerase chain reaction
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要