Deregulated levels of RUVBL1 induce transcription-dependent replication stress

•Both overexpression and depletion of RUVBL1 induce transcription-dependent replication stress, but the underlying mechanisms are different.•RUVBL1 overexpression results in increased c-Myc dependent pause release of RNAPII.•RUVBL1 knock-down leads to accumulation of ubiquitinated RNA polymerases on chromatin, likely due to impaired INO80 remodeler function.