Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting.

Erika Casari,Elisa Gobbini,Michela Clerici,Maria Pia Longhese

HOMOLOGOUS RECOMBINATION: Methods and Protocols（2021）

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摘要

Generation of 3' single-stranded DNA (ssDNA) at the ends of a double-strand break (DSB) is essential to initiate repair by homology-directed mechanisms. Here we describe a Southern blot-based method to visualize the generation of ssDNA at the ends of site-specific DSBs generated in the Saccharomyces cerevisiae genome.

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关键词

DNA double-strand breaks,HO endonuclease,Resection,S. cerevisiae,Single-stranded DNA,Southern blot

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