Quantification of Coronaviruses by Titration In Vitro and Ex Vivo.
Methods in molecular biology (Clifton, N.J.)(2020)
摘要
Several techniques are currently available to quickly and accurately quantify the number of virus particles in a sample, taking advantage of advanced technologies improving old techniques or generating new ones, generally relying on partial detection methods or structural analysis. Therefore, characterization of virus infectivity in a sample is often essential, and classical virological methods are extremely powerful in providing accurate results even in an old-fashioned way. In this chapter, we describe in detail the techniques routinely used to estimate the number of viable infectious coronavirus particles in a given sample. All these techniques are serial dilution assays, also known as titrations or end-point dilution assays (EPDA).
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关键词
CD50,End-point dilution assay (EPDA),Plaque-forming units,TCID50,Titration
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