Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis.

Romualdas Vaisvila, Lise Hunault,Lana Saleh

DNA MODIFICATIONS: Methods and Protocols（2021）

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摘要

The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high throughput capillary gel electrophoresis assay for monitoring the in vitro oxidation of 5mC by TET. The method is rapid and quantitative, and can serve as a powerful tool in mechanistic studies of TET.

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关键词

5-(β-glucosyloxymethyl)cytosine,5-methylcytosine, 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine,Capillary gel electrophoresis,T4 phage β-glucosyltransferase,Ten-eleven translocation dioxygenase

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