Screening Of Toll-Like Receptor Signaling Pathway-Related Genes And The Response Of Recombinant Glutathione S-Transferase A3 Protein To Thiram Induced Apoptosis In Chicken Erythrocytes

The expression of genes related to the Toll-like receptors (TLRs) signaling pathway were determined.Group A, B and C fed with basal diet and group D, E and F induced TD by feeding a basal diet containing 100 mg.kg(-1) thiram. rGSTA3 protein was injected at 20 mu g.kg(-1) in group B, E and at 50 mu g.kg(-1) in C, F.Results suggested that lameness and death of chondrocytes were significant on day 14. TLRs signaling pathway related genes were screened based on the transcriptome enrichment, and validated on qPCR. IL-7, TLR2, 3, 4, 5, 7, 15, MyD88, MHC-II, MDA5 and TRAF6 were significantly (p < 0.05) expressed in group E and F as compared to group D on day 14 and 23. IL-7, MHCII, TRAF6, TLR3, TLR5, TLR7, and TLR15 determined insignificant in group D compared to group A on day 23.TD occur in an early phase and alleviated in the later period. rGSTA3 protein can prevent apoptosis and repair degraded chondrocytes.