818: THE TIME COURSE OF HEAT SHOCK PROTEIN 70 EXPRESSION AND THE RELATIONSHIP TO GLUTAMINE IN THE CRITICALLY ILL CHILD

Introduction: Activation of poly (ADP-ribose) polymerase-1 (PARP-1) contributes to neuronal damage in many neurological insults. Poly-ADP-ribosylation reaction consumes nicotinamide adenine dinucleotide (NAD). NAD serves as an important energy substrate and a co-factor for Complex I (CI) of the electron transport chain. Therefore, PARP-1 activation can result in impaired energy metabolism further exacerbating injury. Whether PARP-1 activation contributes to energy failure in status epilepticus (SE) remains undefined. Hypothesis: PARP-1 activation contributes to NAD depletion, impaired CI respiration and hippocampal neuronal damage following SE. Methods: SE was induced in male rats (100–150 g) using intraperitoneal kainate (15 mg/kg, ip). PARP-1 inhibitor (PJ-34, 15 mg/kg, ip) or vehicle was given prior to SE induction. Animals remained in SE for 1h. Twenty-four hours following SE, hippocampal NAD …