Highly-selective detection of EGFR mutation gene in lung cancer based on surface enhanced Raman spectroscopy and asymmetric PCR
Journal of Pharmaceutical and Biomedical Analysis(2020)
摘要
•By combining asymmetric polymerase chain reaction (PCR) and SERS, a highly-selective detection for EGFR mutation genes in lung cancer was developed.•This combined asymmetric PCR-SERS method had a high detection threshold (4.24 fM).•In the further detection in 15 blood samples, the asymmetric PCR-SERS method had an overall sensitivity of 75% and specificity of 100%.
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关键词
Asymmetric PCR,Circulating tumor DNA,Lung cancer,Surface enhanced Raman scattering,Trace detection
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