Long amplicon sequencing for improved genetic characterization of African swine fever virus

David A Meekins,Jessie D Trujillo,Natasha N Gaudreault,Igor Morozov,Daniel Pérez-Núñez,Yolanda Revilla,Juergen A Richt

Journal of Virological Methods（2020）

引用 4|浏览27

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摘要

•A method was developed to rapidly sequence −40% of the ASFV genome.•Improved genomic coverage/depth was obtained compared to standard characterization.•The primers used are broadly compatible with published whole ASFV genomes.•Provides an improved diagnostic tool for ASFV epidemiological monitoring.

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关键词

African swine fever virus,Next generation sequencing,Molecular epidemiology,Asfarviridae

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