Efficient biotransformation of l-lysine into cadaverine by strengthening pyridoxal 5’-phosphate-dependent proteins in Escherichia coli with cold shock treatment

Biochemical Engineering Journal(2020)

引用 27|浏览3
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摘要
•BL21 harboring pdxY under the J23100 promoter resulted in highest yield of PLP.•pdxK plays a critical role in accumulation of PLP for biotransformation.•Lysine was 100 % converted to cadaverine in co-expressing pdxK and CadA.•Cadaverine biotransformation was enhanced for in vivo and in vitro applications.•Whole-cell biocatalyst achieved the highest cadaverine productivity of 121 g/L/h.
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关键词
Pyridoxal 5’-phosphate,Cadaverine,Biotransformation,Super-salvage pathway,Cold shock treatment
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