Development of an SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus

Journal of Virological Methods(2020)

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摘要
•The first development a SYBR Green I-based real-time PCR assay to detect canine kobuvirus.•Canine kobuvirus (CaKoV) was detected at a rate of 8.7 % in the Anhui province.•This method has implications for the prevention and control of CaKoV-infect in dogs.
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关键词
Detection,Canine kobuvirus,Real-time PCR,SYBR Green I
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