Sensitivity of Bleak ( Alburnus alburnus ) in Detection of the Wastewater Related Pressure in Large Lowland Rivers

This study deals with bleak ( Alburnus alburnus ) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as ”stretch specific”, or it should be linked to the sampling site and characterised as ”site specific”. The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as ”site specific”. Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.