1 1 2 Hybrid Voltage Sensor Imaging from Genetically Targeted Neurons in Hippocampal and 3 Spinal Cord Slices

1 2 Hybrid Voltage Sensor Imaging from Genetically Targeted Neurons in Hippocampal and 3 Spinal Cord Slices 4 5 6 Dongsheng Wang, Tiffany Poon, Shane McMahon, Samuel L. Pfaff, Lea Ziskind7 Conhaim, Baron Chanda, Meyer B. Jackson 8 9 1. Department of Physiology, University of Wisconsin, Madison, WI 53706. 10 2. Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk 11 Institute for Biological Sciences, San Diego, CA 92109. 12 3. Biophysics Ph.D. Program, University of Wisconsin, Madison, WI 53706. 13 Contact information: Meyer Jackson, Dept. Physiology, University of Wisconsin, 14 Madison WI, 53706; mjackson@physiology.wisc.edu 15 16 Running head: Voltage imaging from genetically targeted cells 17 18 19 Abstract 20 Genetically encoded optical voltage sensors function well in cultured cells, but 21 their greatest promise, the study of intact neural circuits, remains unfulfilled. Here we 22 tested genetically encoded probes in brain and spinal slices from transgenic mice using 23