On-chip immunoprecipitation for 1 protein purification †

The UCD community has made this article openly available. Please share how this access benefits you. Your story matters! (@ucd_oa) Some rights reserved. For more information, please see the item record link above. Immunoprecipitation (IP) is one of the most widely used and selective techniques for protein purification. Here, a miniaturised, polymer-supported immunoprecipitation (mIP) method for the on-chip purification of proteins from complex mixtures is described. A 4 ml PDMS column functionalised with covalently bound antibodies was created and all critical aspects of the mIP protocol (antibody immobilisation, blocking of potential non-specific adsorption sites, sample incubation and washing conditions) were assessed and optimised. The optimised mIP method was used to obtain purified fractions of affinity-tagged protein from a bacterial lysate.