atumoral Localization of Aromatase and Interaction een Stromal and Parenchymal Cells in the Non – Small R l Lung Carcinoma Microenvironment

Download ogens produced as a result of intratumoral aromatization has been recently shown to play important n proliferation of human non–small cell lung carcinomas (NSCLC), but the details have remained largely wn. Therefore, in this study, we evaluated the possible roles of intratumoral aromatase in NSCLCs as s: (a) evaluation of intratumoral localization of aromatase mRNA/protein in six lung adenocarcinoma using laser capture microdissection combined with quantitative reverse transcriptase-PCR and immuochemistry; (b) examination of the possible effects of isolated stromal cells from lung carcinoma tissues matase mRNA transcript expression in lung carcinoma cell lines (A549 and LK87) through a coculture ; and (c) screening of cytokines derived from stromal LK001S and LK002S cells using cytokine antibody and subsequent evaluation of effects of these cytokines on aromatase expression in A549 and LK87. romatase mRNA and protein were mainly detected in intratumoral carcinoma cells but not in stromal romatase expression of A549 and LK87 was upregulated in the presence of LK001S or LK002S cells. l cytokines such as interleukin-6 (IL-6), oncostatin M, and tumor necrosis factor-α, all known as inducctors of aromatase gene, were detected in conditioned media of LK001S and LK002S cells. Treatment of ncostatin M and IL-6 induced aromatase gene expression in A549 an LK87, respectively. These results all ted that intratumoral microenvironments, especially carcinoma-stromal cell interactions, play a pivotal indica role in the regulation of intratumoral estrogen synthesis through aromatase expression in human lung adenocarcinomas. Cancer Res; 70(16); 6659–69. ©2010 AACR.