Automation of PacBio SMRTbell 10 kb Template Preparation on an Agilent NGS Workstation

Automation of the PacBio 10 kb Template Preparation using the SMRTbell Template Prep kit for the sequencing of bacterial genomes on the Agilent Automated Liquid Handling Platform is described. Four bacterial genomes of differing GC content are used to prepare libraries without bias reading for next generation sequencing. The gDNA quality assessment was done using spectrophotometric and automated electrophoresis for high molecular weight DNA with an Agilent 2200 TapeStation system with the Agilent Genomic DNA ScreenTape assay. The sheared DNA was measured with the Agilent Bioanalyzer system and the DNA 12000 Kit.