Advanced Fiber Evaluation Workflows

The first two approaches handle resolved fibers in high magnification images. The first method demonstrates how to compute so called local orientations, where the fiber volume is subdivided into blocks of equal size. Each of such blocks will then be transferred into Fourier space in order to compute an orientation of the block’s content. In the second case we will present our recent XFiber extension for tracing the centerline of cylindrical fibers by applying a template matching approach followed by a specific line tracing algorithm. Extracting each individual fiber centreline, this approach enables fiber counting, but also extraction of statistics such as fiber length, orientation or curvature.