An efficient and scalable pipeline for epitope tagging in mammalian stem 2 cells using Cas 9 ribonucleoprotein 3

Pooran, Singh, Dewari, Benjamin, Southgate, Katrina, Mccarten, Monogarov,Eoghan O'Duibhir,Niall Quinn,Ashley Tyrer, Marie-Christin Leitner,Colin Plumb, Maria Kalantzaki,Carla Blin, Rebecca Finch,Raul Bardini Bressan,Gillian Morrison,Ashley M. Jacobi,Mark A. Behlke,Alex Von Kriegsheim,Simon Tomlinson,Jeroen Krijgsveld,Steven M. Pollard

semanticscholar（2018）

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摘要

1MRC Centre for Regenerative Medicine and Edinburgh Cancer Research UK Centre, 11 University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK. 12 2German Cancer Research Center (DKFZ) and the University of Heidelberg, Im 13 Neuenheimer Feld 581, 69120, Heidelberg, Germany. 14 3Integrated DNA Technologies, Inc., 1710 Commercial Park, Coralville, IA, 52241, 15 USA 16 4Edinburgh Cancer Research UK Centre Institute of Genetics and Molecular Medicine 17 (ECRC-IGMM), University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, UK 18

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