Function and mechanism of long non-coding RNA KIAA 0495 in glioma

Objective: To investigate the expression of long non-coding RNA KIAA0495 in human glioma tissues, and the effect of U251 glioma cells on the biological function as well as its mechanism. Methods: We selected 100 cases of glioma specimens and 50 cases of normal brain tissue specimens, then used PCR to detect the expression levels of genes in cells, and used Pearson correlation analysis to detect the expression correlation of KIAA0495 and CTNNBIP1. Results: In glioma, the expression level of KIAA0495 was inversely proportional to the degree of malignant gliomas, HGG (high grade glioma) expression was significantly lower than that of the LGG (Lower grade glioma); correspondingly, the expression of CTNNBIP1 was inversely proportional to the degree of malignant gliomas and the expression level of HGG was lower than that of the LGG. Thus, the analysis of its relevance told us a positive correlation between them. The up-regulation of KIAA0495 expression inhibited cell proliferation, migration, cycle, but promoted cell apoptosis. On the basis of the over-expression of KIAA0495, we interfered the expression of CTNNBIP1, then found that compared with over-expressed KIAA0495 group, the proliferation, migration and cell cycle inhibition were all weakened. And the promoting effect of cell apoptosis was inhibited in some degree. Experiment of subcutaneously transplanted tumor (in vivo) in nude mice confirmed that the down regulation of CTNNBIP1 expression could weaken the inhibitory effect of KIAA0495 on tumor growth. Conclusion: In glioma cells, increasing lncRNA KIAA0495 can slow down the growth of glioma cells, and this inhibition can be achieved by regulating the expression of CTNNBIP1 protein. Thus, this result means that lncRNA KIAA0495 could become a new therapeutic target for glioma.