Evaluation of smooth lipopolysaccharide based latex agglutination test in serodiagnosis of brucellosis

In this study, sLPS antigen coated latex bead was prepared and their evaluation was done along with RBPT, commonly employed for the serodiagnosis of brucellosis. sLPS was extracted from Brucella abortus S99 and coated on polystyrene latex beads in a phosphate buffer saline coating buffer. A total of 200 samples were collected from unorganized dairy farm and tested with RBPT and sLPS based latex agglutination test. The accuracy, relative sensitivity and specificity of sLPS based LAT were found 99%, 93.55% and 100% respectively . This study conclude that sLPS based LAT can be used as an alternative screening test for bovine brucellosis.