Effects of Lipid-Related Factors on Adipocy Vascular Cells in Primary Culture te Differentiation of Bovine Stromal -

observation. Addition of triglyceride mixture (Intralipid), caprylic acid and yery !ow-, lowand high-density lipoproteins (VLDL, LDL and HDL) stirnulated bovine preadipocyte differentiation in seturn-free condition. Especially, VLDL strongly increased both cell protein contents and GPDH activity, suggesting that it stimulated both proliferation and differentiation efbovine preadipocytes, Under Intralipidinduced condition, differcntiation of preadipocytes from subcutaneous adipose tissues was more evident than those from omental adipose tissues. However, such depot difference was not observed in medium supplemented with indomethacin, which is a peroxisome proliferator-activated receptor (PPAR) v agonist, This suggests that the differentiation capacity of bovine preadipocytes was different between depots and such difference is dependcnt on the abi]ity to utilize lipids as endogenous PPARv Iigands. Therefore, lipid metabelites have the stimulatory effects on bovine adipocyte differentiation in vitro, and lipoproteins, e pecially VLDL. rnay p)ay an irnportant role in development of bovine adipose tissues in vivo.