Fingerprinting maps construction of Brassica napus and the rapeseed germplasm with super-high oil content in China

In this study, we selected 7 rapeseed germplasms with super-high oil content and 16 main rapeseed cultivars from the different planted regions to construct their fingerprinting maps by SSR molecular markers. Using 28 SSR primer pairs from the different linkage groups, 302 SSR bands were amplified for the all materials, 279 of which were polymorphism bands, each primer amplified 6-16 bands with 10.79 bands on average, the average of polymorphism rate reached 92.38%. By this way, we could distinguish all the materials better. UPGAM analyzing revealed that there were the large difference among the high oil content germplasms and between the high oil content germplasms and the main cultivars. At genetic distance of 0.171, the 23 B. napus could be divided into nine groups, in which the superhigh oil content germplasms were divided into four groups, and 8 main cultivars were divided into five groups. These results showed that all materials had the most abundant genetic diversity, and there were large genetic differences between the super-high oil germplasms and the main rapeseed cultivars.