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    • Image processing in super-resolution two-photon point-scanning structured illumination microscopy

    E. Slenders,M. Vandeven

    semanticscholar（2017）

    Cited 0|Views1
    Abstract
    Fluorescence microscopy with a lateral spatial resolution beyond the diffraction limit, i.e. superresolution microscopy, is of great interest in cell biology. Unlike other super-resolution modalities, structured illumination microscopy (SIM) is fast and does not require a high illumination power. Furthermore, SIM can be made compatible with two-photon fluorescence by point scanning over the sample object with a focused pulsed laser while temporally modulating the excitation intensity, a crucial step for in vivo super-resolution microscopy [1].
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