Evaluation of toxicity in shsy 5 y cells stably overexpressing lrrk 2

Introduction: Mutations in leucine rich repeat kinase 2, LRRK2, are the most common known cause of genetic Parkinson's disease. Transient overexpression of mutant LRRK2 can cause toxicity in cell culture and primary neurons; however recent reports suggest that this depends on experimental setups. Also, this acute cellular toxicity contrasts with findings from LRRK2 transgenic mice which generally do not display neuronal cell death.