Impact of Microbiological Media on Quality Microbiological Finding

The contemporary society, pressured by globalization, requires accurate and reliable but above all comparable results. An important tool for advering comparability is ensurance of result traceability. The goal of each diagnostic microbiological laboratory, whose work is based on methods of cultivation of bacteria, is to set up fast and reliable microbiological diagnosis. In bacteriology, cultivation and reproduction of MM is the foundation of direct proof of bacteria from samples so the accuracy in preparation of the MM is imperative. Testing laboratories determine control procedures of MM prepared from dehydrated base as well as the ways to control technological processes of preparation according to DIN EN ISO 11133:2014. To prepare MM dehydrated bases that are produced in accordance with the wording of the pharmacopoeia (EP, USP) and reference to international standards (ISO, APHA) are used. The physical parameters that are controlled by the MM are: the pH value, the thickness of the plates, filling volume, color, clarity, consistency. The microbiological parameters that are controlled include sterility and productivity and selectivity of the certified reference material (CRM). CRM contain microorganisms of stable characteristics that are typical for a particular microbial species, and is suitable for proving the effectiveness of a particular MM and was purchased from internationally acknowledged collections. Quality microbiological media is one of the most important factors in the work of microbiological laboratories for bacteriological diagnosis. A proper implementation of the technological process of preparing is one of the key requirements in the system of implementation of quality control of the MM. The ISO/IEC 17025:2007 international standard require from laboratories to evaluate the measurement uncertainties of their results. An excessive measurement uncertainty calls into question the possibility of making the right decision based on these results (it refers to making a precise diagnosis or estimating therapeutic efficacy).