In vitro study on alpha amylase inhibitory activity of murrya koenigii: effect of extraction solvents

Murrya koenigii Linn. Spreng Wettst (M. koenigii L.) is a medicinal plant. In traditional medicine the plant is used to treat leukoderma, piles, stomach problem, kidney pain and blood disorders. Different parts of the plant showed pharmacological activities like anti cancer, anti inflammatory, anti oxidant, anti diabetic, gastro protective, hepato protective, photo-protective anti gastric ulcer, anti microbial etc. The plant also possesses alpha amylase inhibitory activity. Aim of the present work was to check effect of solvent extracts on in vitro alpha amylase inhibitory activity of M. koenigii L. leaves. M. koenigii L. was collected from the local market and identified by the taxonomist. Solvent extractions of the leaves were made separately by using chloroform, petroleum ether, ethanol, isopropanol and hexane. Extracts were separately dried and processed for in vitro alpha amylase inhibitory activity by standard method. Acarbose, an alpha amylase inhibitor, was used as control. Results showed that ethanol extract of M. koenigii L. leaves had maximum alpha amylase inhibitory activity in comparison to that of other solvent extracts. In vitro alpha amylase inhibitory activity of ethanol extract of M. koenigii L. leaves (IC50 value 36.72±1.50 μg/mL) was also comparable to that of acarbose (IC50 value 66.66±1.6 μg/mL). This study therefore indicates uses of ethanol extract of M. koenigii L. leaves in the management of diabetes. World Journal of Pharmaceutical Research SJIF Impa t Factor 8.074 Volume 8, Issue 9, 1232-1240. Research Article ISSN 2277– 7105 Article Received on 08 June 2019, Revised on 29 June 2019, Accepted on 18 July 2019 DOI: 10.20959/wjpr20199-15520 *Corresponding Author