A new in vitro assay measuring direct interaction of nonsense suppressors with the eukaryotic protein synthesis machinery

Nonsense suppressors (NonSups) induce “readthrough”, i.e., the selection of near cognate tRNAs at premature termination codons and insertion of the corresponding amino acid into nascent polypeptide. Prior readthrough measurements utilized contexts in which NonSups can promote readthrough directly, by binding to one or more of the components of the protein synthesis machinery, or indirectly, by several other mechanisms. Here we utilize a new, highly-purified in vitro assay to measure exclusively direct nonsense suppressor-induced readthrough. Of 16 NonSups tested, 12 display direct readthrough, with results suggesting that such NonSups act by at least two different mechanisms. In preliminary work we demonstrate the potential of single molecule fluorescence energy transfer measurements to elucidate mechanisms of NonSup-induced direct readthrough, which will aid efforts to identify NonSups having improved clinical efficacy. ![Figure][1] * AG : aminoglycoside CrPV : cricket paralysis virus IRES : internal ribosome entry site NonSups : nonsense suppressors PTC : premature termination codon smFRET : single molecule fluorescence resonance energy transfer [1]: pending:yes